ASCB follows a rigorous, multi-stage workflow to ensure that every stem cell line deposited in our biobank meets the highest standards of quality, authenticity, and safety before being made available to the research community.
Ethical collection of biological samples from donor animals following CPCSEA and IAEC-approved protocols with full documentation.
Primary cell isolation, culture establishment, and expansion under sterile, standardized laboratory conditions using defined media and substrates.
Comprehensive characterization including mycoplasma testing, karyotyping, STR profiling, sterility assays, and pluripotency marker validation.
Validated cryopreservation using optimized protocols to ensure long-term viability - with backup storage in multiple secure liquid nitrogen tanks.
Detailed metadata capture including species, tissue source, passage number, culture conditions, and characterization reports in our digital database.
Researchers submit formal requests; ASCB reviews eligibility, processes Material Transfer Agreements (MTAs), and obtains necessary regulatory approvals.
Secure packaging and shipment of viable cell lines on dry ice with complete characterization certificates and culture protocols to approved institutes.
Technical assistance for revival, culture optimization, and troubleshooting. Feedback collection to improve repository quality and expand the collection.
A continuous cycle of rigorous testing to ensure every cell line exceeds international standards before repository acceptance.
Ensuring the authenticity, safety, and functional potency of every cell line.
From sample collection to repository-ready stem cell line.
Aseptic tissue harvest and primary extraction.
Establishing stable cell populations under optimal media conditions.
Rigorous testing for markers, safety, and pluripotency.
Cryopreservation in LN2 and database integration.
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Designed and Developed by Venkata Avinash
Technical Assistant